Legacy to Legato®: KD Scientific Syringe Pump Selection Made Simple

Selecting the right syringe pump is essential for ensuring precision and reliability in fluid handling applications. Cole-Parmer offers a broad range of KD Scientific syringe pumps tailored to meet laboratories' evolving needs, from simple infusion to sophisticated multi-step protocols. This article compares the Legacy KDS 100, Legato® 100, and Legato® 200 series syringe pumps, outlining the key differences, ideal applications, and tips for getting the most out of your system.

KD Scientific Pump Series Overview

Legacy Series KDS 100

The Legacy KDS 100 is the foundation of KD Scientific’s long-standing reputation for combining precision and simplicity. It offers reliable infusion functionality with essential features, including a backlit LCD for clear visibility and an intuitive setup process. Users can easily configure the flow rate, select syringe size, and set dispense volume, making it simple to tailor the pump to specific application needs. Volume and flow rate units (µL/hr or mL/hr) are selected automatically based on syringe size. The KDS 100 remains a dependable and cost-effective choice for straightforward infusion applications where simplicity and reliability are top priorities.

Legato Series

The Legato series represents the next generation of syringe pump innovation, combining superior flow performance with intuitive usability and advanced connectivity. The high-resolution 4.3" color touchscreen display is a standout feature, which shows all pump operating parameters on a single screen including, flow rate, volume delivered, elapsed time, time remaining, and syringe selection. Core features like USB, TTL, and RS-485 communication enable seamless integration with PCs and laboratory automation systems. Daisy-chain up to 99 pumps for synchronized multi-pump workflows. Unique to the Legato design, the screen orientation rotates automatically when placed on its side, reducing the pump’s footprint by four times to maximize valuable bench space. The adjustable linear force mechanism ensures optimal pressure is applied to a wide range of syringe sizes, while the rugged welded steel chassis minimizes vibration and noise, even during high-pressure operation enhancing stability and performance. The Legato 100 series delivers these essential capabilities in a cost-effective platform ideal for single- or dual-syringe infusion and infuse/withdraw applications with excellent precision and flow stability.

Engineered for performance and flexibility, the Legato 200 series builds on the capabilities of the Legato 100, making it ideal for demanding research and industrial environments. The 200 series accommodates larger syringe volumes and includes RS-232 communication for expanded system integration. With higher linear force, these pumps are well-suited for viscous fluids and high-pressure applications. Integrated features like calibration reminders, dual external triggers, and a real-time clock display make this series a go-to choice for complex, high-precision workflows.

How to Choose the Right Pump

Selecting the right syringe pump starts with identifying your application's key requirements. Considering these factors will help narrow down your options and ensure optimal performance and usability.

Number of Syringes

• Single syringe pumps are ideal for simple tasks where only one reagent or fluid needs to be handled at a time.
• Dual syringe pumps allow simultaneous or alternating functions, enabling continuous flow or precise volume matching.

Programmable

• Basic control with no programming offers straightforward operation, making them ideal for fixed-rate applications where parameters remain consistent throughout the process.
• Limited programming allows users to create and store two custom programs with up to 50 steps each, providing flexibility for simple, repeatable workflows.
• Advanced programmability offers the most robust programming options, including a single complex program with up to 100 steps or storage for 40 programs with 25 steps each. This is ideal for high-precision, multi-phase protocols or regulated environments requiring method validation.

Syringe Size

• Smaller syringes provide higher precision for nanoliter/picoliter flows, while larger syringes support high-volume transfers and longer run times.
• Use the smallest viable syringe size for higher flow control resolution.

Cole-Parmer’s Syringe Pump Selection

Cole-Parmer offers a comprehensive selection of KD Scientific syringe pumps. Utilize these factors to match your application needs with the right model for optimal performance, accuracy, and workflow efficiency.

Item Number	Model	# of Syringes	Mode	Programmable	Syringe Size	Flow Rate	Voltage
51005-10	Legacy KDS 100	1	Infusion	No	10 µL to 60 mL	1667 pL/min to 7.05 mL/min	110 VAC
51005-11	Legacy KDS 100		Infusion	No	10 µL to 60 mL	1667 pL/min to 7.05 mL/min	230 VAC
51005-01	Legato 100		Infusion	No	0.5 µL to 60 mL	1.28 pL/min to 88.28 mL/min	100–240 VAC
51005-03	Legato 110		Infusion/Withdrawal	2 Programs/50 steps each	0.5 µL to 60 mL	1.28 pL/min to 88.28 mL/min	100–240 VAC
51005-06	Legato 185		Infusion/Withdrawal	2 Programs/50 steps each	0.5 µL to 60 mL	0.54 pL/min to 39 mL/min	100–240 VAC
51005-02	Legato 101	2	Infusion	No	0.5 µL to 10 mL	1.28 pL/min to 25.99 mL/min	100–240 VAC
51005-04	Legato 111		Infusion/Withdrawal	2 Programs/50 steps each	0.5 µL to 10 mL	1.28 pL/min to 25.99 mL/min	100–240 VAC
51005-05	Legato 180		Infusion/Withdrawal	2 Programs/50 steps each	0.5 µL to 10 mL	0.58 pL/min to 11.70 mL/min	100–240 VAC
51005-07	Legato 200		Infusion	No	0.5 µL to 140 mL	3.06 pL/min to 215.8 mL/min	100–240 VAC
51005-08	Legato 210		Infusion/Withdrawal	1 Program/100 steps	0.5 µL to 140 mL	3.06 pL/min to 215.8 mL/min	100–240 VAC
51005-09	Legato 210P		Infusion/Withdrawal	40 Programs/25 steps	0.5 µL to 140 mL	3.06 pL/min to 215.8 mL/min	100–240 VAC

Essential Supporting Components

For optimal performance and compatibility, consider these accessories:

Real-World Applications and Recommended Models

Application	Ideal Pump	Reason
Electrospinning or MS Calibration	Legacy KDS 100 or Legato 100	Basic infusion functionality with reliable flow and easy setup.
Surface Plasma Resonance, Microdialysis	Legato 101	Dual syringe configuration and nanoliter flow precision for low-volume applications.
Dosing or Nutrient Delivery	Legato 110 or 111	Infuse/withdraw modes with programmable steps for precise dosing control.
Microfluidics, Ultra-Low-Flow Delivery	Legato 180	Picoliter flow rates with high reproducibility for exact applications.
Cell Injections, Neuroscience	Legato 185	Single-syringe picoliter delivery for delicate, high-precision dosing.
Drug Discovery, Flow Chemistry	Legato 200	Supports larger syringe volumes and viscous fluids.
Biopharma R&D	Legato 210	Advanced control, multi-mode operation, and compliance-friendly features.
Organic Synthesis, Complex Dosing	Legato 210 or 210P	Multi-step programmability and event-triggered operations for automated workflows.

Tips for Long-Term and Optimal Use

• Routine Calibration: Legato 200 models feature reminders and diagnostics for timely calibration and maintenance.
• Syringe Accuracy: Measure the syringe bore diameter to enter precise syringe dimensions. Use the entry option to input the actual value in millimeters (mm) to optimize flow rate calculations.
• Mechanical Maintenance: Regularly inspect and lubricate the guide rods and lead screw to maintain smooth motion and extend pump life.
• Linear Force Adjustment: Set the appropriate linear force based on syringe size and fluid viscosity to prevent stalling or syringe damage.

Select with Confidence

From reliable infusions to complex operations, KD Scientific pumps offered by Cole-Parmer support a wide range of applications in the lab and beyond.

Explore Cole-Parmer’s complete syringe pump portfolio or contact our technical specialists to find the best solution for your application needs.