Sartorius Sartobind® Lab IDA Metal Affinity Membrane Adsorber, 2.1 mL; 2/PK

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Sartorius Mfr # 93IDA-42DB-12--V – Item # RK-64822-71
Optimize your affinity purification for purity and yield using the metal ion of your choice
  • Higher flow rates compared with IDA resins
  • Constructed with a stabilized reinforced cellulose membrane and polypropylene housing

$402.24CAD / Pkg of 2

SPECS & DESCRIPTION

Specifications & Description

  • Membrane MaterialStabilized reinforced cellulose
  • Pore Size (micron)3
  • Filter Size25 mm Diameter Membrane
  • Housing MaterialPolypropylene
  • ConnectionsFemale Luer Inlet x Male Luer Outlet
  • Capacity (mL)2.1
  • Effective Filtration Area (cm²)75
  • Typical Water Flow Rate10 to 63 mL/min
  • Max Pressure (PSI)87
  • DescriptionSartobind® Lab IDA Metal Affinity Membrane Adsorber, 2.1 mL; 2/PK

Key Features

  • Higher flow rates compared with IDA resins
  • Constructed with a stabilized reinforced cellulose membrane and polypropylene housing

More About this Item

Sartorius Sartobind® Lab membrane adsorbers for immobilized metal affinity chromatography (IMAC) are ready-to-use units in a syringe filter format. They are based on the patented macroporous Sartobind membranes, with iminodiacetic acid (IDA) covalently bound to the entire internal surface of the pores. This results in a chromatography matrix with superior flow rates and high binding capacity. The IDA ligand enables coupling of the metal ion of your choice to the membrane adsorber, ensuring simple process optimization for maximum purity and yield of each his-tagged protein. The Sartobind Lab IDA adsorber is available with a 2.1 mL bed volume, providing binding capacities up to 7.5 mg his-tagged protein per unit. Equipped with standard Luer lock connectors, Sartobind Lab units enable simple, equipment-free handling by syringe, or can be easily connected to FPLC systems for automated purifications. The units are compatible with most common chromatography buffers and can be regenerated for multiple purification cycles, ensuring high-cost efficiency.

Sartobind Metal Chelate adsorbers represent a new generation of Immobilized Metal Affinity Chromatography (IMAC) purification devices. They can simply be used in an HPLC, FPLC or operated by hand with a syringe connected via Luer Lock. The iminodiacetic acid (IDA) ligand is attached to the inner surface of a membrane which is fitted into a filter holder for easy and quick handling, making His-tagged protein purification nearly as easy as filtration. Sartobind Metal Chelate units are designed to simplify the chromatographic steps normally associated with IMAC. This makes them also a convenient and quick tool for screening purposes.

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